常用载体及相关通用引物 |
载体 |
一端 |
另一端 |
pBlueScriptSK(+) |
M13F(-77)/M13F(-47)/M13F/T7 |
M13R(-88)/M13R(-48)/M13R/T3 |
pcDNA3 |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
BGH |
pcDNA3.1(+) |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.0(+)/myc-His C/A/B |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
BGH |
pcDNA3.1(+)/myc-His C/A/B |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1(+)/myc-His/LacZ |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/His C/A/B |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/His/LacZ |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/Hygro(-) |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/Hygro(+) |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/Hygro/LacZ |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/V5-His C/A/B |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/V5-His/LacZ |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/V5-His-TOPO |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/V5-His-TOPO/LacZ |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/Zeo(-) |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/Zeo(+) |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pcDNA3.1/Zeo/CAT |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
pCDNA3.1R/BGH |
pColdI DNA |
p-GEX3’ |
|
pColdII DNA |
p-GEX3’ |
|
pColdIII DNA |
p-GEX3’ |
|
pColdIV DNA |
p-GEX3’ |
|
pDream2.1 |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
SP6 |
pEGFP-N1,2,3 |
H1.3F/pCMV-F /pEGFP-N-5 |
pEGFP-N-3’/pEGFP-C-5’/pEGFP-C-3' |
pET15b |
T7 |
T7ter |
pET20b |
T7 |
T7ter |
pET22b |
T7 |
T7ter |
pET28a |
T7 |
T7ter |
pET28b |
T7 |
T7ter |
pET30b |
T7 |
T7ter |
pFastBac Dual |
pEGFP-C-3' |
|
pFastBac HT A,B,C |
pEGFP-C-3' |
|
pFastBac1 |
pEGFP-C-3' |
|
pGEM-T Easy |
M13F(-77)/M13F(-47)/M13F/T7 |
M13R(-88)/M13R(-48)/M13R |
pGEX-4T-1/3 |
p-GEX5’ |
p-GEX3’ |
pGS-21a |
p-GEX5’ |
T7ter |
pLenti6/V5-D-TOPO |
M13F(-47)/M13F/T7 |
M13R(-48)/M13R/T3 |
pLenti6/V5-GW/lacZ |
M13F(-47)/M13F/T7 |
M13R(-48)/M13R/T3 |
pMAL-p2x |
M13F(-47)/M13F/PBV220R |
M13R(-48) |
pMD18-T |
M13F(-77)/M13F(-47)/M13F |
M13R(-88)/M13R(-48)/M13R |
pMD19-T |
M13F(-77)/M13F(-47)/M13F |
M13R(-88)/M13R(-48)/M13R |
pMTBiPV5-His GFP |
p-GEX3’/ M13F(-47)/M13F |
M13R(-88)/M13R(-48)/M13R /pEGFP-C-3'/BGH |
pPCR script |
M13F(-77)/M13F(-47)/M13F/T7 |
M13R(-88)/M13R(-48)/M13R/T3 |
pPIC3.5 |
5’AOX |
3’AOX |
pPIC9 |
5’AOX |
3’AOX |
pPICZ C/A/B |
5’AOX |
3’AOX |
pPICZalpha C/A/B |
5’AOX/α-Factor |
3’AOX |
pSecTag2 A,B,C |
H1.3F/pCMV-F /pEGFP-N-5/T7 |
BGH |
pTWIN |
T7 |
T7ter |
pUC57 |
M13F(-77)/M13F(-47)/M13F |
M13R(-88)/M13R(-48)/M13R |
pUC18 |
M13F(-47)/M13F |
M13R(-48)/M13R |
pUC19 |
M13F(-47)/M13F |
M13R(-48)/M13R |
其他 |
…… |
…… |
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| 注：1）排列顺序为：有左到右==（离插入为点）由远到近； |
| 2）若有多个引物，红色的为首选引物： |
| 3）选择测序通用引物时，以距离插入位点约50碱基为佳； |
| 4）同时有T7，T7ter的载体，单反应首选T7ter； |
| 5）同时有T7，pCDNA3.1R的载体，单反应首选pCDNA3.1R ； |
| 6）同时有T7，BGH的载体，单反应首选BGH； |
| 7）同时有pCMV-F，BGH的载体，单反应首选BGH； |
测序通用引物
