DNA Sequencing

RealGene Research has already been proofed their advanced skills and techniques in the areas of biotechnology, genetic engineering and molecular biology. Bulk orders (96-well plates formatted):

The price will be going down depending on the amount of plates.
These samples will be formatted into each well of the plates as follow:
Add 4ul of template DNA, 150ng-160ng/ul, must be correct concentration!!!
Add 2ul of 2.5-5uMole of a primer.
Total volume in each well is 6ul, then, seal the plates tightly with strip caps or very thick gel heat-sealer films.
Note: In the sky when air-shipping, the air pressure will be reduced, leaking out and under the film will make samples lost and contaminations between wells.
High through-put capillary sequencers. Read more than 900 bases.

• Turn-around time: two to three business days usually (P.R.Chinese)
• Data reported electronically via e-mail

Files delieved: ABI Electropherogram and Simple Text
How to Order Sequencing Services

• Order by three ways

1. For single order, you need to fill the Sequencing Sample Order Form here (MS Excel format), print out the order form, send us a copy of the form together with your samples, and at the same time send us an E-mail with order form attached (electronic copy).


2. For orders located in ShangHai, if you have more than 20 samples per day, we can arrange a pick-up service for you, please call us at (0086-21) 54460771 ， you can also send us an inquiry via fax (0086-21)54460772.
3. For bulk orders and contract services, quotations may be needed The price per reaction is negotiable

• Sequencing Sample Order Form (MS Excel format)

Primers Used for Sequencing

• Universal and often-used primers , provided without extra charge in RealGene Sequencing Lab.
• Primers not on the list or gene specific primers (GSP) should be prepared by customers and submitted together with sequencing samples. Required concentration and amount of submitted Primers.

. Table: Ready4U Primers *