Viral Vector Services
RealGene has long experience in gene synthesis and cloning which give us an edge in the area of gene expression.Now we offer clients one centralized source of expertise, involving the design, creation, and production of viral vectors for gene expression and transfer. Our services offer adenoviral (AV), adeno-associated viral (AAV), lentiviral (LV), and retroviral (RV) vectors.
RealGene has completed hundreds of viral vector requests each year since the department began to work. We continually update our standard operating procedures to incorporate new techniques and technologies into our viral vector Services.Our viral vector service comprised of solid, safe and proven techniques and technology.
Key Features:
- Diverse vector systems
- Professional vector construction
- Full consultation services
- Human and mouse cDNA libraries associated
Viral Vector Selection Guide:
RealGene offers four viral vector systems. The genome size, packaging capacity, limitations, and other important features of the four viral vectors are summarized in the table below.
Viral Vector |
Viral Genome |
Packing Capacity |
Tropism |
Inflammatory Potential |
Limitations |
Advantages |
Adenovirus
(Non-enveloped) |
dsDNA |
8-40 kb |
Broad |
High |
Capsid mediates a potent inflammatory response |
Efficient transduction of
most tissues |
AAV
(Non-enveloped) |
ssDNA |
4.7 kb |
Broad, with possible exception of haematopoietic cells |
Low |
Small packaging capacity |
Non-inflammatory;
non-pathogenic |
Lentivirus
(Enveloped) |
RNA |
8 kb |
Broad |
Low |
Potential oncogenesis induction |
Persistent gene transfer
in dividing cells |
Retrovirus
(Enveloped) |
RNA |
8 kb |
Dividing cells only |
Low |
Transduces only dividing cells, potential oncogenesis induction |
Persistent gene transfer
in dividing cells |
 Viral Vector Construction Procedures:
Select a viral vector that efficiently transduces target cells without affecting cell viability. In some cases, it is recommended that a pilot study be performed using a vector expressing eGFP or galactosidase reporter.
Clone the gene of interest and the selected promoter into appropriate shuttle vectors and verify transgene insertion into the shuttle vector by PCR and sequencing.
Confirm the infectivity of the recombinant virus by transfection assay, and verify transgene expression by ELISA or immunoblot. Where possible, it is also advisable to show that the transgene product functions as required before proceeding to virus packaging and preparation.
Quotations and Ordering:
For quotation requests, please download and complete RealGene Viral Vector Services Quotation Request Form, and email or fax it to us with a detailed description of project requirements. For questions, please contact us by email at service@realgene.com.cn, phone, or fax.Orders can be placed by phone, email, or fax with either a formal PO (Purchase Order) or credit card. Please email or fax us a completed RealGene Viral Vector Services Quotation Request Form. If target gene is physically available, please submit your target gene along with a hard copy of completed RealGene Viral Vector Services Quotation Request Form to us. |
